MedLabSTAT: Histology Histology, MedLabStat Share This Post Which stain uses the silver substitution method? Day 13: February 24th, 2022 Answer: Von Kossa stain is used to detect presence of calcium. Ca ions are substituted with Ag ions giving a black appearance. PrevPreviousMedLabSTAT: Transfusion NextMedLabSTAT: CHemistryNext Search for Related Posts Search Search Report Resource Form Leave a Reply Cancel replyYou must be logged in to post a comment.